OspB, is one of the major outer surface proteins of the outer membrane of borrelia burgdorferi, which is composed of various unique outer surface proteins (Osp) that have been characterized (OspA through OspF).
The Osp proteins are lipoproteins anchored by N-terminally attached fatty acid molecules to the membrane.
They are presumed to play a role in virulence, transmission, or survival in the tick.
Two of the major surface Ag of borrelia burgdorferi, the 31kD OspA and 34kD OspB proteins, show a high degree of sequence similarity, are encoded by a 49kb plasmid and share a common promoter, and are coordinately transcribed.
OspA, OspB, and OspD are expressed by B.
burgdorferi residing in the gut of unfed ticks, suggesting that they promote the persistence of the spirochete in ticks between blood meals.
OspB has a contributing role in the adherence of B.
burgdorferi to the tick gut.
The C-terminal of OspB is important for eliciting a protective immune response to OspB.
B.
burgdorferi has the ability to vary its surface proteins in response to immune attack.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:13,000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes Borrelia burgdorferi OspB.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：MBP-fusion protein corresponding to Borrelia burgdorferi OspB.
