Outer-Surface Protein A (OspA), a lipoprotein from borrelia burgdorferi encoded on its plasmid lp54, is a major component of the spirochete's extracellular matrix.
OspA probably serves as a lipid-anchor.
The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host.
This transition may be facilitated by changes in expression of some B.
burgdorferi genes.
Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change.
It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host.
B.
burgdorferi can attach to (and also differentially express) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B.
burgdorferi gene expression.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes Borrelia burgdorferi OspA.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：MBP-fusion protein corresponding to Borrelia burgdorferi OspA.
