NRF1(also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles.
In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-a) gene.
This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
NRF1 forms a homodimer and binds DNA as a dimer.
NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues.
Phosphorylation of NRF1 enhances DNA binding.
Multiple splice variants have been identified for this protein.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:3000-1:10,000Western Blot: 1:500-1:2000Immunohistochemistry: 2mg/ml-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes mouse NRF1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa1-534 from mouse NRF1.
