84-2135-08　NFYA (Nuclear Transcription Factor Y Subunit alpha, Nuclear Transcription Factor Y Subunit A, NF-YA, CAAT-box DNA-binding Protein Subunit A, FLJ11236) 100ul　168393

特徴

• Nuclear transcription factor Y subunit A is a protein that in humans is encoded by the NFYA gene.
• The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes.
• Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity.
• The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit.
• In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation.
• Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
• NF-Y is ideal for investigators involved in transcription factor research.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:20,000-1:100,000GelShift: Use 2ulWestern Blot: 1:2000-1:10,000Immunohistochemistry: 1:400Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.