NFKB was originally identified as a factor that binds to the immunoglobulin k-light chain enhancer in B cells.
It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFKB bound to I?B.
NF?B was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits.
Other identified subunits include p52 (NFKB2), cRel, and RelB.
The p65, cRel, and RelB subunits are responsible for transactivation.
The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate.
p52 has been reported to form transcriptionally active heterodimers with the NF?B subunit p65, similar to p50/p65 heterodimers.
Low levels of p52 and p50 homodimers can also exist in cells.
The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by I?B-a.
I?B-a ?binds to the p65 subunit preventing nuclear localization and DNA binding.
Activators mediate a rapid phosphorylation of I?B by I?B kinase (IKK) which results in subsequent ubiquitination and proteolytic degradation.
NF?B is then transported to the nucleus, where it activates transcription of target genes through binding to NF?B target sequences within the promoter.
The HTLV-I protein Tax can induce constitutive NF?B activation through phosphorylation of both I?B-a and I?B-B.
The transforming protein tax inhibits p53 transcriptional activity through the NF?B signaling pathway, specifically via the p65 (RelA) subunit.
The inhibition of p53 activity is dependent upon phosphorylation of p65 (RelA) at S536 by the upstream kinase IKKB.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:25,000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Prepared from monospecific antiserum by delipidation and defibrination.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human NFkB p65 phospho S276. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to to a region near phospho Serine 276 of the human protein conjugated to KLH.
