Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of a-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates.
They are widely spread in nature, occurring in viruses, bacteria, fungi, protozoa, birds and mammals.
Together, the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs.
Three types of neuraminidase are found in mammals and are defined as lysosomal, plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution.
Lysosomal N-acetyl-a-neuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity.
However, unlike other members of this family, lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation.
The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight, multi-protein complex containing PPCA, B-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase.
Using a hamster Sial3 probe, Monti et al.
(1999) identified the gene encoding sialidase-2, which they designated NEU2, from a human genomic library.The 2 putative exons of NEU2 encode a deduced 380aa protein with a calculated molecular mass of 42.23kD.
The NEU2 protein has significant homology with the mammalian, viral, and bacterial sialidases.
It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with human NEU1.
NEU2 contains a potential N-linked glycosylation site, 2 aspartic acid block consensus sequences, and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes.
Monti et al.
hypothesized that NEU2 has a cytosolic localization because it does not contain a cleavage site, transmembrane domain, or targeting motifs.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Neu2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa110-124 from human Neu2.
