Microtubules are polymers of tubulin, which exist as heterodimers of a-tubulin and b-tubulin.
NEDD1(neural precursor expressed, developmentally down-regulated protein1; also called GCP-WD) is a centrosomal protein that in mammals associates with the g-tubulin ring complex (?-TuRC).
?-TuRC is critical for initiation, or nucleation, of the microtubule assembly.
In association with this complex, NEDD1 plays an important role in targeting the ?-TuRC complex to the site of microtubule nucleation and to the mitotic spindle.
These events are essential for proper bipolar spindle formation and mitotic progression.
Given the casual link between improper spindle function and tumorigenesis, characterization of Nedd1 function will be important to better understand various mechanisms underlying mitotic regulation, chromosome segregation, and cancer development.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000Western Blot: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human NEDD1. Species sequence homology: Turkey, chicken, salmon, and danio (91%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ch Hu
Immunogen：Recombinant protein corresponding to the aa343-667 from human Nedd1.
