The NCOA3 (also known as nuclear receptor coactivator 3 isoform a) is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions in a hormone-dependent fashion.
The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex.
NCOA3 probably acts via remodeling of chromatin and is involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:175,000Western Blot: 1:500-1:1000Immunoprecipitation: 1:100Immunohistochemistry (Paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human NCOA3. Reacts with several isoforms of human NCOA3 protein (a, b, d, e and f).
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein.
