In general, myosins are protein complexes consisting of one or more myosin heavy chains, associated light chains and other proteins.
Myosins function as molecular motors and use the energy of ATP hydrolysis to move actin filaments or to move vesicles or other cargo on fixed actin filaments.
Myosins have magnesium-ATPase activity and bind actin.
Myosins can be divided into classes that are distinguished based on sequence features of the motor, or head domain, but also have distinct tail regions that are believed to bind specific cargoes.
Unconventional myosins exist.
Myosin 1G is an unconventional myosin that is restricted to hematopoietic cells.
Unconventional myosins are also critical for motility in amoeba and a mammalian paralog (Myo1C) is critical as a glucose transporter that recycles glucose in response to insulin.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:40,000Western Blot: 1:500-1:3000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Myosin 1G. Species Crossreactivity: mouse, chimpanzee and rat.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Myosin 1G.
