MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals.
It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling.
MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression.
It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS.
This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription.
At least three isoforms of MPYS are known to exist.
Applications:Suitable for use in Immunocytochemistry, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Immunocytochemistry: 5ug/mlELISA: 1:10,000-1:40,000Western Blot: 1-2ug/ml, a band of ~37-42kD is expectedOptimal dilutions to be determined by the researcher.
Positive Control:A20 cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human MPYS. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide near the C-terminus of human MPYS.
