Myeloid leukemia factor-1 (MLF1) Interacting protein, also known as PBIP1, is a novel gene that encodes a putative transcriptional repressor.
It is localized to human chromosome 4q35.1, is expressed in both the nuclei and cytoplasm of cells, and is a novel substrate for polo-like kinase (PLK1).
Phosphorylation by PLK1 regulates the PLK1-MLF1 interaction and localization to the kinetochores promoting proper chromosome segregation.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:650,000Western Blot: 1:500-1:2000, expect a band ~65kD corresponding to MLF1IP protein in the appropriate cell lysate or extract
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human MLF1IP.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human MLF1IP at C-terminus. Species sequence homology: chimpanzee, equine, bovine, canine, mouse, rat and chicken
