84-2134-91　MLF1 (HHV8 LNAIP1, ICEN24, Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1, KLIP-1, KLIP1, KSHV latent nuclear antigen interacting protein 1) 100ug　168372

特徴

• Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate.
• Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition.
• Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry.
• Mutations of these sites stabilize MLF1IP and inhibit mitotic progression.
• Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation.
• Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block.
• Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset.
• MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation.
• Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,400,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.