MIP1-A and MIP1-B were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line.
Human MIP1-B refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a.
The predicted protein products of these cDNAs represent variants that are between 94% 98% identical and these proteins are all approximately 75% homologous to murine MIP1-B.
MIP1-B also shares approximately 70% aa identity with MIP1-A.
MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after mitogen stimulation.
The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1-A and MIP1-B preferentially attracting CD8+ and CD4+ T cells, respectively.
A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1-A, MIP1- B, MCP1 and RANTES with varying affinities has been isolated.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000-1:100,000Western Blot: 1:2000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O or PBS prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human MIP-1B.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human MIP-1B.
