MIP-3a (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3a, MIP-3-a, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes.
MIP-3a inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.
C-terminal processed forms have been shown to be equally chemotactically active for leukocytes.
MIP-3a also possesses bacterial activity against E.coli and S.aureus.
MIP-3a is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung.
Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon.
C-terminal processed forms which lack 1, 3 or 6aa are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000-1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes mouse MIP-3a. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to full length mouse MIP-3a.
