MIP-1A and MIP-1B were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line.
Human MIP-1B refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a.
The predicted protein products of these cDNAs represent variants that are between 94%-98% identical and these proteins are all approximately 75% homologous to murine MIP-1B.
MIP-1B also shares approximately 70% aa identity with MIP-1A.
MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after mitogen stimulation.
The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP-1A and MIP-1B preferentially attracting CD8+ and CD4+ T cells, respectively.
A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP-1A, MIP-1 B, MCP1 and RANTES with varying affinities has been isolated.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes human MIP-1B.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human MIB-1B.
