84-2134-75　MDM2 (E3 ubiquitin-protein ligase Mdm2 EC=6.3.2.- p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein) 100ug　168354

特徴

• MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90kD that forms a complex with the p53 tumor suppressor protein.
• Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2).
• The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor.
• Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53-mediated transactivation.
• MDM2 binds to p53 and amplification of MDM2 in sarcomas leads to escape from p53-regulated growth control.
• This mechanism of tumorigenesis parallels that for virus-induced tumors in which viral oncogene products bind to and functionally inactivate p53.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:3000-1:12,000Western Blot: 1:500-1:2000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.