MCP-1, or Monocyte chemoattractant protein 1, of the intercrine b (chemokine CC) family, is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils.
It augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis.
It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
MCP-1 exists as a monomer or homodimer; in equilibrium.
It binds to CCR2 and CCR4 and is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.
Processing at the N-terminus can regulate receptor and target cell selectivity.
Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes human MCP-1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human MCP-1 expressed in E.coli.
