This recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses.
MARK2 refers to MAP/microtubule affinity- regulating kinase 2 isoform a [Homo sapiens].
EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer.
Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2.
These isoforms were characterized by the presence of a 162bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it.
Both forms were found to be co-expressed in a number of selected cell lines and tissue samples.
Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed.
This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein.
Multiple splice variants exist for this protein.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:3000Western Blot: 1:250-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human MARK2, phosphorylated at T595. Species Crossreactivity: canine, bovine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to rabbit MARK2, phosphorylated at T595.
