Lysozyme is a relatively small (129aa) secretory enzyme that catalyzes the hydrolysis of B-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers.
Lysozyme is common in animals and plants.
In birds, lysozyme is also an exceptionally abundant protein in egg whites.
Its biological function in fowl eggs is unclear.
Hen egg white lysozyme ''c'' is the most commonly studied form and source of the enzyme.
Lysozyme from domestic goose is designated lysozyme ''g''.
Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacteria susceptible to osmotic lysis.
Plant lysozyme is found in ficus and papaya latex, and is chemically distinct from the egg white enzyme.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:15,000-1:75,000Western Blot: 1:500-1:5000Immunohistochemistry: 1:500-1:2000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：chicken
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes hen lysozyme.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Ch
Immunogen：Native full length hen egg white lysozyme.
