ORF57 (also known as MTA), one of the earliest Kaposi's sarcoma-associated herpesvirus (KSHV) regulatory proteins to be expressed, is essential for virus lytic replication.
A counterpart is present in every herpesvirus sequenced, indicating the importance of this signature viral protein, and those examined act post-transcriptionally, affecting RNA splicing and transport.
KSHV ORF57 is capable of establishing both lytic and latent replication cycles.
In KS, the virus localizes to tumor progenitor endothelial cells, most of which are latently infected.
In cell culture, KSHV replication is generally studied using B-cell lines, such as BCBL-1, generated from primary effusion lymphoma material.
Most BCBL-1 cells are latently infected, although there is some spontaneous virus reactivation.
Addition of chemical inducers such as sodium n-butyrate, 12-O-tetradecanoylphorbol-13-acetate (TPA), and valproic acid (VA) to these cells efficiently induces the lytic cycle and produces virions.
KSHV ORF57 protein predominantly localizes to the nucleus and can shuttle between the nucleus and cytoplasm.
Most HSV-1 genes are unspliced; by contrast, ORF57 is spliced gene; the protein is 455aa in length and 50kD in size.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000-1:170,000Western Blot: 1:5000-1:8000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human KSHV ORF57.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rb
Immunogen：Synthetic peptide corresponding to human KSHV ORF57 at C-terminus.
