KLH, peroxidase conjugated, detects keyhole limpet hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000.
KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons.
Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2).
When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color.
The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein.
The large and highly glycosylated KLH protein cannot be reproduced synthetically.
It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata.
KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:400,000Western Blot: 1:1000-1:5000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP). Do not add azide.
