KLH detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000.
KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons.
Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2).
When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color.
The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein.
The large and highly glycosylated KLH protein cannot be reproduced synthetically.
It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata.
KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:1000-1:5000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
