Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR).
IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-kB and MAP kinase (MAPK) by IL-1R/TLR.
A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4.
Overexpression of IRAK-4 activates NF-kB and MAPK pathways.
IRAK-4 interacts with and phosphorylates IRAK-1.
IRAK-4-deficient animals are completely resistant to challenge with LPS.
Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges.
Members of the IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:250-1:1000, a band of ~50kD is expectedImmunohistochemistry: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human IRAK-4. No cross reactivity occurs against other IRAKs.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rb
Immunogen：Synthetic peptide corresponding to a region near the C-terminus of human IRAK-4.
