IRAK-M (Interleukin-1 receptor-associated kinase M).
Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induce cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR).
Through IL-1/Toll receptors, IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-kB; a pivotal transcription factor mediating inflammatory and immune response.
A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M.
IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-kB activation.
Members of the IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:500-1:2000Immunohistochemistry: 1:300-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from whole serum.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human IRAK-M. Species Crossreactivity: mouse and rat. No crossreaction with IRAK or IRAK2.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids from the c-terminus of human IRAK-M.
