Interleukin 13 (IL-13), of the IL-4 superfamily, is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease.
IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ra) and at least one IL-13-specific binding chain.
Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, STAT6.
In humans, IL-13 can induce immunoglobulin E (IgE) secretion from activated B cells.
In mice, deletion of IL-13 does not markedly affect either Th2 cell development or specific IgE responses induced by potent allergens.
Deletion of IL-4 abrogates these responses.
IL-13 acts as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thus altering physiological function.
Although IL-13 is associated primarily with the induction of airway disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, it also induces airway matrix metalloproteinases as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation.
IL-13 is ideal for investigators involved in Cancer and Immunology research.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:11,000Western Blot: 1.5-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS.
Specificity：Recognizes porcine IL-13. Species Crossreactivity: bovine and sheep.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Po Sh
Immunogen：Recombinant protein corresponding to full length swine IL-13.
