Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs.
Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes.
IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1.
Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages.
IL-34 is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:40,000Western Blot: 0.25-0.5ug/ml, a band of ~48kD is expectedOptimal dilutions to be determined by the researcher.
Positive Control:Human brain tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from whole serum
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
Specificity：Recognizes human IL-34. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a region near the N-terminal of human IL-34
