IL-32A (also known as natural killer cells protein 4, tumor necrosis factor a-inducing factor, IL32a, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family.
IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses.
The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence.
Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2.
This protein induces the production of TNFa and IL-8.
It induces typical cytokine pathways of NF-kB and p38 MAPK.
Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000-1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Reconstitute with sterile dH2O or PBS. Labeled with Biotin.
Specificity：Recognizes human IL-32A.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human IL-32A.
