The cytokine Interleukin 27 (IL-27) is produced in response to inflammation.
It is made by activated presenting cells including monocytes, endothelial cells, and dendritic cells.
IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A).
It is a regulator of T helper cell development and suppressor of T-cell proliferation.
IL-27 has both pro- and inflammatory properties.
It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells.
It has diverse effects on innate immune cells.
It induces monocytes and mast cells to secrete pro-inflammatory cytokines.
When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses.
As an inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses.
The IL-27 p28 subunit, a 28kD glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6.
The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34kD glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family.
It can exist as a homodimer and can also heterodimerize with IL-12 p35.
It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.
EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer.
The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130.
WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells.
Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines.
IL-27 also promotes effector functions of NK cells and CD8+ T cells.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:1000-1:5000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes mouse IL-27/p28.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to full length mouse IL-27/p28.
