IL-1B (also known as Interleukin-1 b, IL-1B and catabolin) is produced by activated macrophages.
IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.
IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
IL-1B is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:1000-1:5000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt.
Reconstitute with sterile dH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes human IL-1B. Species Crossreactivity: Primate and canine.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Hu
Immunogen：Recombinant protein corresponding to human IL-1B expressed in E.coli.
