IL-1B (also known as Interleukin-1B, IL-1B and catabolin) is produced by activated macrophages.
IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.
IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
IL-1B is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
IL-1B is ideal for investigators involved in cardiovascular and immunology research.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:250ELISA: 1:1000-1:5000Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:250Immunoprecipitation: 1:200-1:800Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2. Reconstitute with sterile dH2O.
Specificity：Recognizes mouse Il-1B. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC IP WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to mouse IL-1B expressed in E.coli.
