IL-1 b (also known as Interleukin-1b IL-11b and catabolin) is produced by activated macrophages.
IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.
IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
IL-1B is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
IL- B is ideal for investigators involved in cardiovascular and immunology research.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:5000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Highly Purified
Purity:Purified by DEAE chromatography.
Form:Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2. Reconstitute with sterile dH2O or PBS.
Specificity:Recognizes human IL-1B. Species Crossreactivity: Primate and canine.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Ca Hu
Immunogen:Recombinant protein corresponding to human IL-1B expressed in E.coli.