Interleukin-18 (IL-18) is a member of the IL-1 cytokine family and was initially identified as an Interferon-g (IFN-g) inducing factor (IGIF).
The IL-18 gene was originally cloned from liver cells and has since been shown to be produced by activated monocytes/macrophages, Kupffer cells, keratinocytes, glucocorticoid-secreting adrenal cortex cells, osteoblasts and dendritic cells.
IL-18 is a 24kD, non-glycosylated polypeptide that lacks a classical signal sequence and possesses a structure recognizably similar to IL-1.
IL-18 is synthesized as a bio-inactive propeptide that undergoes proteolytic cleavage by either ICE (interleukin-1b converting enzyme) or another caspase to generate a mature, bioactive, 18kD molecule.
In both the mature and propeptide forms, IL-18 shows 64% aa sequence identity from mouse to human.
IL-18 does not appear to show any primary sequence similarity to any other known cytokines.
Rat IL-18 has also been isolated, and found to be 194aa in length with a 91%aa sequence identity to mouse IL-18.
Human IL-18 has been found to induce the production of IFN-g and GM-CSF while inhibiting the production of IL-10 by PBMC.
With respect to human T cells, IL-18 enhances Th1 cytokine production and stimulates cell proliferation via an IL-2-dependent pathway.
Human IL-18 can also inhibit the synthesis of IgE by B cells.
Thus, IL-18 plays an important role in immunological and inflammatory reactions.
Currently, the bioactivity of human IL-18 is often determined by its capacity to augment the levels of IFN-g produced by T cells as measured in tissue culture supernatants.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:5000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2.
Specificity：Recognizes mouse Il-18.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to 157aa from mouse IL-18 expressed in E.coli.
