84-2133-81　HMGN, phosphorylated (S20/S24) (High mobility group nucleosome binding domain containing protein 1, High mobility group protein 14, High-mobility group nucleosome binding domain 1, HMG14) 100ug　168245

特徴

• HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences.
• Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis.
• The sequence immunized is conserved in several species.
• As such, this reagent is designed as a ''universal'' reagent for the detection of all phosphorylated HMGN proteins.The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels.
• HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin.
• HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family.
• Each HMG family has a characteristic functional sequence motif.
• The functional motif of the HMGB family is called the ''HMG-box;'' that of the HMGN family, the ''nucleosomal binding domain;'' and that of the HMGA family, the ''AT-hook.'' The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms.
• Proteins containing any of these functional motifs embedded in their sequence are known as ''HMG motif proteins.''
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:5000-1:25,000Western Blot: 1:500-1:2000Immunohistochemistry: 20ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.