84-2133-74　HA Epitope Tag, YPYDVPDYA, Hemagglutinin (HRP) 100ug　168234

特徴

• Epitope tags are short peptide sequences that are easily recognized by tag-specific.
• Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.
• Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
• This allows epitope tag to serve as universal detection reagents for any tag containing protein produced by recombinant means.
• This means that epitope tags are a useful alternative to generating specific to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
• The epitope tag is usually functional in a variety of dependent experimental procedures.
• Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:15,000Western Blot: 1:1000-1:5000Immunohistochemistry: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile dH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Labeled with horseradish peroxidase (HRP).
• Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• HRP conjugates are sensitive to light.