Green fluorescent protein is a 27kD protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light.
GFP is an important tool in cell biology research.
GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Applications:Suitable for use in Immunofluorescence and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1:2500Flow Cytometry: 1:2000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 0.01% sodium azide, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with fluorescein isothiocyanate (FITC).
Specificity：Recognizes GFP.
Isotype：IgG
Calc Applications Abbrev：FC IF
Immunogen：Recombinant protein corresponding to full length GFP derived from the jellyfish Aequorea victoria.
