Green fluorescent protein is a 27kD protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light.
GFP is an important tool in cell biology research.
GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
GFP is ideal for Cell Biology, Neuroscience and Cancer research.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:24,000-1:100,000Western Blot: 1:500-1:5000Immunohistochemistry: 1:200-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity:Recognizes GFP.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Immunogen:Recombinant protein corresponding to full length GFP from jellyfish Aequorea victoria.