GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology.
GST will react with synthetic construct present in most known GST containing cloning or expression vectors.
GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
The aa sequence GST is highly conserved in most organisms including mammals.
GST exists as a 26kD homodimer.
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting.
This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Applications:Suitable for use in Immunofluorescence and FLISA.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Rhodamine conjugates are sensitive to light.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 0.01% sodium azide, 10mg/ml BSA. Reconstitute with sterile dH2O or PBS. Labeled with Rhodamine.
Specificity：Recognizes GST.
Isotype：IgG
Calc Applications Abbrev：E FLISA IF
Immunogen：Full length GST isolated from Schistosoma japonicum.
