GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology.
GST will react with synthetic construct present in most known GST containing cloning or expression vectors.
GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
The aa sequence GST is highly conserved in most organisms including mammals.
GST exists as a 26kD homodimer.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:10,000Western Blot: 1:500-1:2500Immunohistochemistry: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.05M tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 0.01% sodium azide, 50% glycerol, 10mg/ml BSA. Labeled with Alkaline Phophatase (AP).
Specificity：Recognizes GST.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Immunogen：Native full length GST isolated from Schistosoma japonicum.
