Glutathione Peroxidase 4 (GPx4, also known as PHGPx) functions and also plays a role in redox regulation, sexual maturation, inflammation, apoptosis, and differentiation.
GPx4 was found to have an important role in preventing lipid peroxidation, and has been discussed primarily as the glutathione peroxidase protecting biomembranes against oxidative stress.
GPx4 can react with free hydrogen peroxide as well as with a wide range of lipid hydroperoxides, including those derived from cholesterol and cholesteryl esters.
GPx4 is the only GPx to use phospholipid hydroperoxides as substrates.
It can also reduce thymine hydroperoxide.
In contrast to GPx1 and GPx3, GPx4 can use a wide range of reducing substrates in addition to glutathione.
GPx4 is expressed in high levels in testes, suggesting a role in male fertility.
It has also been identified as an enzymatically inactive structural protein of the mitochondrial capsule of sperm.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:7000-1:30,000Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes mouse Glutathione Peroxidase 4. Species Crossreactivity: rat and guinea pig.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Gp Mo Rt
Immunogen：Synthetic peptide corresponding to mouse GPx4 at C-terminus.
