Gli-3 (also known as zinc finger protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc finger protein family and contains 5 C2H2-type zinc fingers.
Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal.
Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.
Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and cranio-facial development.
Two isoforms of human Gli-3 have been reported.
One is the full-length protein at~170-190kD and the other is a truncated isoform at~80kD.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:6000-1:30,000Western Blot: 1:500-1:2000Immunohistochemistry: 0.5mg/ml-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Gli-3. Species Crossreactivity: chimpanzee, squirrel monkey, xenopus laevis, chicken, canine and quail. Species sequence homology: mouse (94%) and rat (88%).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Ch Hu Mk Xe
Immunogen：Synthetic peptide corresponding to aa41-57 from human Gli-3.
