Gli-2 (also known as zinc finger protein Gli-2, GLI-Kruppel family member GLI-2 or tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family.
Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs.
These motifs contain conserved H-C links.
Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell.
The protein encoded by this gene localizes to the cytoplasm and activates patched drosophila.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:12,000Western Blot: 1:500-1:2000Immunohistochemistry: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Gli-2. Species Crossreactivity: chimpanzee.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa46-60 from human Gli-2 (isoform a).
