GGA1 (also known as ADP-ribosylation factor binding protein, golgi-localized, g-ear-containing, ARF-binding protein 1, G-adaptin related protein 1) plays a role in protein sorting and trafficking between the trans-golgi network (TGN) and endosomes.
This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
GGA1 is a monomeric protein that interacts with NECAP1, GGA2 and GGA3 as well as RABEP1, RABGEF1 and type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1.
GGA1 is a ubiquitously membrane-associated protein that is localized to TGN and endosomes.
Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:500-1:3000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human GGA-1.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa336-352 from human GGA-1.
