Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-b (TGF-b) superfamily.
NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF).
NAG-1 is expressed in human placenta, prostate and colon.
It possesses antitumorigenic and proapoptotic activities.
NAG-1 expression is dramatically increased in inflammation, injury and malignancy.
Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate.
In a number of studies, NAG-1 expression was increased by a number of NSAIDs.
This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers.
NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein.
Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth.
Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis.
NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1.
EGR-1 may be necessary for NSAID-induced NAG-1 expression.
The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000-1:120,000Western Blot: 1:3000-1:7000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes mouse Nag-1. Species Crossreactivity: human.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to mouse NAG-1 at C-terminal.
