Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-b (TGF-B) superfamily.
NAG-1 is also known as macrophage inhibitory cytokine-1(MIC-1), growth differentiation factor 15 (GDF15), placental bone morphogenetic protein (PLAB), or prostate derived factor (PDF).
NAG-1 is expressed in human placenta, prostate and colon.
It possesses antitumorigenic and proapoptotic activities.
NAG-1 expression is dramatically increased in inflammation, injury and malignancy.
Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate.
In a number of studies, NAG-1 expression was increased by a number of NSAIDs.
This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers.
NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein.
Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth.
Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis.
NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1.
EGR-1 may be necessary for NSAID-induced NAG-1 expression.
The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in 0.02M PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Reconstitute with sterile dH2O or PBS. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human NAG-1. Species Crossreactivity: mouse. Species sequence homology: macaque (94%), rat (70%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human NAG-1 expressed in E.coli, MBP-tagged.
