Flagellin is a protein found in the hollow cylinder forming the filament in bacterial flagellum.
Its structure is helical, which is important for its function.
Studies comparing aflagellate borrelia to flagellated indicate that the flagella have a role in the invasion of human tissue.
The N- and C-terminal of flagellin form the inner core of the flagellar filament, and the central portion of the protein makes up the outer surface.
While the terminus of the protein is quite similar between all bacterial flagellins, the central portion is variable.
The flagellin genes are highly conserved among the different borrelia species.
Mammals often have acquired immune responses (T-cell responses)to flagellated bacterium.
Some bacteria are able to switch between multiple flagellin genes in order to evade this response.
Borrelia burgdorferi, the spirochete that is associated with lyme disease, may use this tactic when challenging mammals with infection.
Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids, in low copy number.
This suggests that initiation of DNA replication and partitioning are carefully controlled during the cell division cycle.
It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host, i.e., changes in environment as the spirochete migrates from the tick's midgut to its salivary glands to the mammal host.
B.
burgdorferi can attach to (and also differentially express in)diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B.
burgdorferi gene expression.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:6000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.01% sodium azide. Reconstitute with 100ul sterile ddH2O.
