84-2133-29　FIV Matrix Protein p15 (Gag polyprotein, Core polyprotein, FIV Matrix Protein p15) 100ug　168181

特徴

• Feline immunodeficiency virus (FIV) belongs to the lentivirus family.
• This family is characterized by assembly of the viral capsid at either the plasma membrane or at the limiting membrane of late endosomes.
• The capsid assembles from the viral Gag polyprotein.
• Upon release of a budding virion, Gag precursor protein is cleaved by the viral protease into its mature products, namely Matrix Protein, Capsid and Nucleocapsid.
• Matrix Protein, located at the N-terminal of the Gag polyprotein, is usually myristylated during protein translation, prior to the later events of virus assembly.
• The myristate moiety is believed to be sequestered within the Matrix Protein during protein translation and later facilitates membrane binding upon exposure resulting from conformational changes.
• Essential functions attributed to the Matrix Protein of lentiviruses include targeting newly synthesized Gag precursor proteins to the site of virus assembly by binding with cellular components such as phosphatidylinositides.
• In the mature virus particle, the Matrix Protein provides internal structure to the virion within the capsid, but is not exposed at the surface of the particle.
• Based on studies with HIV, it is postulated that FIV Matrix Protein may also serve additional functions, including nuclear localization of the viral core upon entry of the virus into a new host cell.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.