The SET domain is a highly conserved, approximately 150aa motif implicated in the modulation of chromatin structure.
It was originally identified as part of a larger conserved region present in the drosophila trithorax protein and was subsequently identified in the drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived.
Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.
ESET functions as a histone methyltransferase by methylation of 9aa of histone H3.
H3 9aa methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones.
ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of condensed chromatin.
Although ESET is a widely expressed protein, it is highly expressed in the testis.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1:1000-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human ESET.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa1058-1075 from human ESET.
