Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers.
The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system.
Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an A-terminal truncated gene product.
Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems.
This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex.
There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes.
Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:90,000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes mouse Eif3S6 Int6. Species Crossreactivity: human, canine, bovine, monkey and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to mouse EIF3S6/Int6 at C-terminus.
