EGR-1(also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site).
EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation.
EGR-1 is a nuclear protein induced by growth factors.
Expression has been identified in a variety of cancers.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000-1:16,000Western Blot: 1:500-1:3000Immunohistochemistry: 220ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human EGR-1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa94-108 from human EGR-1.
