EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development.
Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors.
For example, the epidermal growth factor receptor (EGFR) is mutated and/or overexpressed in many common solid human squamous cell carcinomas including breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium.
Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification.
EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis.
EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2, ErbB-3/HER-3 and ErbB-4/HER-4.
EGFR can heterodimerize with each of the members of this family.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000-1:20,000Western Blot: 1:250-1:1500Immunohistochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human EGFR Phospho Y1197.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rb
Immunogen：Synthetic peptide corresponding to aa1189-1199 from human EGFR.
